Coding
ArtE

Part:BBa_K1659088:Design

Designed by: Wei Chung Kong   Group: iGEM15_Oxford   (2015-08-28)

Artilysin Art-E, an antibacterial fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 342
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes and Sources

We obtained the SMAP-29 peptide sequence from [1] and converted it into a nucleotide sequence using IDT's Codon Optimization Tool, setting the product type for gBlocks Gene Fragments and using the codon table for E. coli K12. The sequence for T4 Endolysin was obtained from BBa_K112806, where nucleotide 20 onwards (the sequence coming after the start codon) was fused downstream of the SMAP-29 nucleotide sequence.

A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.



References

[1] Tack, B.F. et al., 2002. SMAP-29 has two LPS-binding sites and a central hinge. European Journal of Biochemistry, 269(4), pp.1181–1189.